Cliff I. Stains

Associate Professor
Associate Professor Profile Image

Department of Chemistry
University of Nebraska-Lincoln
818C Hamilton Hall
Lincoln, NE 68588-0304
(402) 472-2617


Postdoctoral, MIT
Ph.D., University of Arizona
B.S., Millersville University

Research Interests

Chemical biology, supramolecular chemistry, directed evolution, fluorescence, bioprobes, cell signaling

Current Research

Our laboratory conducts research at the interface of chemistry and biology. We are particularly interested in employing chemical tools and design principles to investigate biological systems. Our research program is centered on three areas involving: 1) new methodologies for constructing designer signaling networks, 2) fundamental studies of protein misfolding, and 3) the development and application of bioprobes. The long term goal of these projects is to provide fundamental insights into biological processes. These multidisciplinary efforts are supported by a combination of approaches including synthesis, protein design and evolution, bioanalytical chemistry, and molecular biology.

Research Overview

For more information, please visit the Stains Research Group Homepage

Selected Publications

(1) Beck, J. R.; Lawrence, A.; Tung, A.; Harris, E. N. & Stains, C. I. Interrogating Endogenous Phosphatase Activity with Rationally Designed Chemosensors. ACS Chem. Biol. 11, 284-290 (2016). [Link]

(2) Zhao, J.; Nelson, T. J.; Vu, Q.; Truong, T. & Stains, C. I. Self-Assembling NanoLuc Luciferase Fragments as Probes for Protein Aggregation in Living Cells. ACS Chem. Biol. 11, 132-138 (2016). [Link]

(3) ) Xu, B.; Zhou, X. & Stains, C. I. Supramolecular Assembly of an Evolved Miniprotein Host and Fluorogenic Guest Pair. J. Am. Chem. Soc. 137, 14252-14255 (2015). [Link]

(4) Beck, J. R.; Zhou, X.; Casey, G. R. & Stains, C. I. Design and Evaluation of a Real-Time Activity Probe for Focal Adhesion Kinase. Anal. Chim. Acta 897, 62-68 (2015). [Link]

(5) Kelly, M. I.; Bechtel, T. J.; Reddy, D. R.; Hankore, E. D.; Beck, J. R. & Stains, C. I. A Real-Time, Fluorescence-Based Assay for Rho-Associated Protein Kinase Activity. Anal. Chim. Acta 891, 284-290 (2015). [Link]

Complete list of publications