Bruker Avance III-HD 700 MHz

Bruker 700 MHz NMR

A brand new Bruker Avance III-HD 700 MHz spectrometer is the latest addition to the RIF. The instrument features a quadruple resonance QCI-P cryoprobe (1H, 13C, 15N, 31P with 2H lock and decoupling) and a SampleJet automated sample changer. This is the top of the line instrument in a center that also features a dedicated solids instrument @ 600 MHz, a 500 MHz NMR with cryoprobe (metabolomics) and instruments at 400 MHz (graduate) and 300 MHz (undergraduate) primarily for small molecule characterization.

This new spectrometer, installed in December 2013 greatly increases sensitivity for metabolomics research and will support fundamental studies in such broad areas as chemical synthesis, molecular evolution, signaling pathways, metabolism and other cellular processes.

The 700 MHz Avance III-HD NMR features 32 shims, which significantly improves line-shape by enhancing field homogeneity across the sample. In addition, the Bruker 700 MHz features state-of-the-art software, allowing for non-uniform data sampling (NUS) with attendant improvements in sensitivity and spectral resolution. NUS dramatically decreases experimental time for multidimensional NMR experiments. A complete set of standard triple-resonance NMR experiments used to assign a protein structure that would normally require a month of dedicated NMR instrument time is reduced to a single day with the new instrument.

The SampleJet automated sample changer that can accommodate over 500 NMR samples for HTS (high throughput screening). Additionally, an internal NMR thermometer that directly measures and continuously the actual sample temperature in real time. Since multidimensional NMR experiments actually lead to sample heating and since peak position, line-shape and field homogeneity are all temperature dependent, the NMR thermometer significantly improves the accuracies of signal frequencies.

Bruker Avance 600 MHz NMR


The 600 MHz Bruker AVANCE spectrometer is a 5 channel NMR system capable of doing both solids and liquids NMR.  It has triple-axis gradient enabling us to perform sophisticated gradient experiments involving multiple nuclei.  The instrument has full-broadband capabilities and a Variable Temperature unit for performing high and low temperature experiments.  This system has a very good selection of probes available for special projects.  The system has a Bruker BCU 05 cooling unit that enhances our ability to run samples near room temperature with good temperature stability.  The 14.7 Tesla magnet provides the higher resolution that is frequently required for running complex biological molecules.
Probes available:

  • 5 mm Broadband Observe with Z Gradients
  • 5 mm Broadband Inverse with Z Gradients
  • 5 mm H/F
  • 5 mm TXI (1H, 13C, 15N) with XYZ Gradients
  • 4 mm CP-MAS Solids



The 500 MHz NMR spectrometer features a 5 mm 1H/13C/15N cryoprobe with a cold proton channel. In Cryoprobes the detection coil is held near liquid Helium temperatures and the proton preamp is held near the temperature of liquid Nitrogen. Holding these two components at these temperatures dramatically reduces the noise floor of the system.  The probe delivers approximately 5 times the proton sensitivity of a room temperature probe.  The system excels at direct detection of proton and as a platform for indirect detection of 13C or 15N through the normal suite of 2-D experiments.  The instrument is normally fitted with a 5 mm Cryoprobe with triple-resonance capabilities, allowing detection of low concentration samples as well as fast acquisition of 2D and 3D datasets for both small molecules and proteins. The addition of the BACS-120 sample changer allows for high-throughput metabolomics screenings and acquisition of submitted samples during evenings and weekends. The instrument supports limited variable-temperature operation with this cryo-probe.
Probes available:

  • 5 mm Triple-resonance Cryoprobe (1H, 13C, 15N) with a Z axis gradient
  • 5 mm QNP, capable of 1H/19F/13C/31P with a Z axis gradient
  • 5mm Triple-resonance (1H, 13C, 15N) Inverse probe with XYZ Gradients

Bruker Avance 400 MHz NMR


The 400 MHz NMR is our most active walk-up system meeting the sample analysis needs of many of the synthetic organic research groups.  The QNP probe is most often used in this instrument, allowing direct observation of 1H, 13C, 31P and 19F with a single probe.  The system has Z-axis gradients and is capable of running Variable Temperature experiments.
The 400 MHz NMR is capable of doing LC-NMR.  An Agilent 1100 HPLC enables researchers to separate their compounds, and the effluent is either stored in a loop fraction collector or injected into the flow probe to run NMR on the samples.  The microcell flow probe is capable of handling quantities of 120 ml.  The 400 NMR is also equipped with the Gilson 215 accessory, which is able to handle up to 96 samples and is extremely useful in combinatorial chemistry.
Probes available:

  • 5mm QNP, capable of 1H/19F/13C/31P
  • 5mm Broadband Observe with gradients
  • 5 mm Broadband Inverse with gradients
  • Flow Probe/3mm Broadband Inverse

Bruker 300 MHz NMR: Nuclear Magnetic Resonance Spectroscopy

Bruker 300

This instrument, installed in May 2006, is available for undergraduate students to use for teaching labs and research. The instrument is jointly shared between the Research Instrumentation Facility and the UIC. Reservations for the system can be made online, or by contacting Sara.

This instrument is currently equipped with a Z-gradient QNP probe that is able to detect 1H/13C/31P/19F without changing any probe settings or cable routing. It also has full-broadband capabilities and a VT unit for performing high and low temperature experiments. Primarily students use the instrument in organic lab to identify products made in the lab by acquiring 1H or 13C data. The new NMR system is able to perform much more complex experiments as well, such as DEPT, COSY, HMQC and solvent suppression. The added nuclei will also make the system more useful in the P-chem and Inorganic labs.