Ph.D. University of Arizona
B.S. Millersville University
Chemical Biology, Directed Evolution, Split-Protein Reassembly, Biomolecular Interactions, Cell Signaling
Our laboratory conducts research at the interface of chemistry and biology. We are particularly interested in employing chemical tools and principles to investigate cell migration and cellular signaling pathways. We are actively developing strategies for: 1) labeling proteins with fluorescent molecules, 2) controlling biological processes with precise spatial and temporal resolution, 3) engineering fluorescent proteins, and 4) quantifying signaling flux through biologically important pathways. These efforts are supported by a combination of multidisciplinary approaches including protein evolution, synthesis, and molecular biology. Read more here.
For more information, please visit the Stains Research Group Homepage.
Stains, C. I.; Tedford, N. C.; Walkup, T. C.; Luković, E.; Goguen, B. N.; Griffith, L. G.; Lauffenburger, D. A. & Imperiali, B. Interrogating Signaling Nodes Involved in Cellular Transformations using Kinase Activity Probes. Chem. Biol. 19, 210-217 (2012).
Stains, C. I.; Luković, E. & Imperiali, B. A p38a-Selective Chemosensor for use in Unfractionated Cell Lysates. ACS Chem. Biol. 6, 101-105 (2011).
Stains, C. I.*; Furman, J. L.*; Porter, J. R.; Rajagopal, S.; Li, Y.; Wyatt, R. T. & Ghosh, I. A General Approach for Receptor and Antibody-Targeted Detection of Native Proteins Utilizing Split-Luciferase Reassembly. ACS Chem. Biol. 5, 943-952 (2010).
*These authors contributed equally to the work.
Stains, C. I.; Furman, J. L.; Segal, D. J. & Ghosh, I. Site Specific Detection of DNA Methylation Utilizing mCpG-SEER. J. Am. Chem. Soc. 128, 9761-9765 (2006).
Stains, C. I.; Porter, J. R.; Ooi, A. T.; Segal, D. J. & Ghosh, I. DNA Sequence-Enabled Reassembly of the Green Fluorescent Protein. J. Am. Chem. Soc. 127, 10782-10783 (2005).
Cliff I. Stains
An undergraduate, graduate, or postdoctoral student in my group can expect to receive training in:
For more information, please visit the Stains Research Group website.
If you are seeking an exciting graduate career that helps you develop research skills at the interface of chemistry and biology, and are a self-motivated and creative individual, please email a letter of interest or resume to Prof. Stains at email@example.com@unl.edu.