University of Nebraska-Lincoln
409D Hamilton Hall
Lincoln, NE 68588-0304
Our laboratory conducts research at the interface of chemistry and biology. We are particularly interested in employing chemical tools and principles to investigate cell migration and cellular signaling pathways. We are actively developing strategies for: 1) labeling proteins with fluorescent molecules, 2) controlling biological processes with precise spatial and temporal resolution, 3) engineering fluorescent proteins, and 4) quantifying signaling flux through biologically important pathways. These efforts are supported by a combination of multidisciplinary approaches including protein evolution, synthesis, and molecular biology.
(1) Stains, C. I.; Tedford, N. C.; Walkup, T. C.; Luković, E.; Goguen, B. N.; Griffith, L. G.; Lauffenburger, D. A. & Imperiali, B. Interrogating Signaling Nodes Involved in Cellular Transformations using Kinase Activity Probes. Chem. Biol. 19, 210-217 (2012).
(2) Stains, C. I.; Luković, E. & Imperiali, B. A p38a-Selective Chemosensor for use in Unfractionated Cell Lysates. ACS Chem. Biol. 6, 101-105 (2011).
(3) Stains, C. I.*; Furman, J. L.*; Porter, J. R.; Rajagopal, S.; Li, Y.; Wyatt, R. T. & Ghosh, I. A General Approach for Receptor and Antibody-Targeted Detection of Native Proteins Utilizing Split-Luciferase Reassembly. ACS Chem. Biol. 5, 943-952 (2010).
*These authors contributed equally to the work.
(4) Stains, C. I.; Furman, J. L.; Segal, D. J. & Ghosh, I. Site Specific Detection of DNA Methylation Utilizing mCpG-SEER. J. Am. Chem. Soc. 128, 9761-9765 (2006).